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Thermostability of a recombinant G protein-coupled receptor expressed at high level in mammalian cell culture

Rational design of pharmaceutical drugs targeting integral membrane G protein-coupled receptors (GPCR) requires thorough understanding of ligand binding and mechanism of activation through high resolution structural studies of purified proteins. Due to inherent conformational flexibility of GPCR, st...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Yeliseev, Alexei, van den Berg, Arjen, Zoubak, Lioudmila, Hines, Kirk, Stepnowski, Sam, Williston, Kyle, Yan, Wanhua, Gawrisch, Klaus, Zmuda, Jonathan
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2020
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7546613/
https://ncbi.nlm.nih.gov/pubmed/33033368
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-73813-7
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