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Application of Strep-Tactin XT for affinity purification of Twin-Strep-tagged CB(2), a G protein-coupled cannabinoid receptor
Human cannabinoid receptor CB(2) belongs to the class A of G protein-coupled receptor (GPCR). High resolution structural studies of CB(2) require milligram quantities of purified, structurally intact protein. Here we describe an efficient protocol for purification of this protein using the Twin-Stre...
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| Publicat a: | Protein Expr Purif |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2016
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5406253/ https://ncbi.nlm.nih.gov/pubmed/27867058 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2016.11.006 |
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