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Application of HaloTag Technology to Expression and Purification of Cannabinoid Receptor CB(2)

Expression of milligram quantities of functional, stable G protein-coupled receptors (GPCR) for high-resolution structural studies remains a challenging task. The goal of this work was to evaluate the usefulness of the HaloTag system (Promega) for expression and purification of the human cannabinoid...

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Detalhes bibliográficos
Main Authors: Locatelli-Hoops, Silvia, Sheen, Fangmin C., Zoubak, Lioudmila, Gawrisch, Klaus, Yeliseev, Alexei A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3627748/
https://ncbi.nlm.nih.gov/pubmed/23470778
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2013.02.011
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