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Application of HaloTag Technology to Expression and Purification of Cannabinoid Receptor CB(2)
Expression of milligram quantities of functional, stable G protein-coupled receptors (GPCR) for high-resolution structural studies remains a challenging task. The goal of this work was to evaluate the usefulness of the HaloTag system (Promega) for expression and purification of the human cannabinoid...
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| Main Authors: | , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2013
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3627748/ https://ncbi.nlm.nih.gov/pubmed/23470778 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2013.02.011 |
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