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Use of Dual Affinity Tags for Expression and Purification of Functional Peripheral Cannabinoid Receptor

The human peripheral cannabinoid receptor (CB2) was expressed as a fusion with the maltose-binding protein (at the N-terminus), thioredoxin A (at the C-terminus) and two small affinity tags (a Strep-tag and a polyhistidine tag). Expression levels of the recombinant receptor in Escherichia coli BL21(...

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Detalhes bibliográficos
Main Authors: Yeliseev, Alexei, Zoubak, Lioudmila, Gawrisch, Klaus
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1906715/
https://ncbi.nlm.nih.gov/pubmed/17223358
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2006.12.003
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