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Expression of human peripheral cannabinoid receptor for structural studies

Human peripheral-type cannabinoid receptor (CB2) was expressed in Escherichia coli as a fusion with the maltose-binding protein, thioredoxin, and a deca-histidine tag. Functional activity and structural integrity of the receptor in bacterial protoplast membranes was confirmed by extensive binding st...

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Autors principals: Yeliseev, Alexei A., Wong, Karen K., Soubias, Olivier, Gawrisch, Klaus
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2005
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2253291/
https://ncbi.nlm.nih.gov/pubmed/16195551
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.051550305
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