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Thermostability of a recombinant G protein-coupled receptor expressed at high level in mammalian cell culture

Rational design of pharmaceutical drugs targeting integral membrane G protein-coupled receptors (GPCR) requires thorough understanding of ligand binding and mechanism of activation through high resolution structural studies of purified proteins. Due to inherent conformational flexibility of GPCR, st...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Sci Rep
Hauptverfasser: Yeliseev, Alexei, van den Berg, Arjen, Zoubak, Lioudmila, Hines, Kirk, Stepnowski, Sam, Williston, Kyle, Yan, Wanhua, Gawrisch, Klaus, Zmuda, Jonathan
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7546613/
https://ncbi.nlm.nih.gov/pubmed/33033368
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-73813-7
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