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Efficient In Vivo Introduction of Point Mutations Using ssODN and a Co-CRISPR Approach
BACKGROUND: The generation of point mutations is a major tool for evaluating the roles of specific nucleotides or amino acids within the regulatory or functional landscape. However, examination of these mutations in vivo requires the generation of animals carrying only the relevant point mutations a...
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| Опубликовано в: : | Biol Proced Online |
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| Главные авторы: | , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
BioMed Central
2020
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7362497/ https://ncbi.nlm.nih.gov/pubmed/32684853 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12575-020-00123-7 |
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