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Efficient In Vivo Introduction of Point Mutations Using ssODN and a Co-CRISPR Approach

BACKGROUND: The generation of point mutations is a major tool for evaluating the roles of specific nucleotides or amino acids within the regulatory or functional landscape. However, examination of these mutations in vivo requires the generation of animals carrying only the relevant point mutations a...

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Dettagli Bibliografici
Pubblicato in:Biol Proced Online
Autori principali: Levi, Tgst, Sloutskin, Anna, Kalifa, Rachel, Juven-Gershon, Tamar, Gerlitz, Offer
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7362497/
https://ncbi.nlm.nih.gov/pubmed/32684853
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12575-020-00123-7
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