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Efficient In Vivo Introduction of Point Mutations Using ssODN and a Co-CRISPR Approach
BACKGROUND: The generation of point mutations is a major tool for evaluating the roles of specific nucleotides or amino acids within the regulatory or functional landscape. However, examination of these mutations in vivo requires the generation of animals carrying only the relevant point mutations a...
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| Publicado en: | Biol Proced Online |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
BioMed Central
2020
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7362497/ https://ncbi.nlm.nih.gov/pubmed/32684853 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12575-020-00123-7 |
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