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Efficient In Vivo Introduction of Point Mutations Using ssODN and a Co-CRISPR Approach

BACKGROUND: The generation of point mutations is a major tool for evaluating the roles of specific nucleotides or amino acids within the regulatory or functional landscape. However, examination of these mutations in vivo requires the generation of animals carrying only the relevant point mutations a...

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Podrobná bibliografie
Vydáno v:Biol Proced Online
Hlavní autoři: Levi, Tgst, Sloutskin, Anna, Kalifa, Rachel, Juven-Gershon, Tamar, Gerlitz, Offer
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7362497/
https://ncbi.nlm.nih.gov/pubmed/32684853
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12575-020-00123-7
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