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ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
The CRISPR-Cas system is a powerful tool for generating genetically modified animals; however, targeted knock-in (KI) via homologous recombination remains difficult in zygotes. Here we show efficient gene KI in rats by combining CRISPR-Cas with single-stranded oligodeoxynucleotides (ssODNs). First,...
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| Publié dans: | Nat Commun |
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| Auteurs principaux: | , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Nature Publishing Group
2016
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4736110/ https://ncbi.nlm.nih.gov/pubmed/26786405 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms10431 |
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