ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

The CRISPR-Cas system is a powerful tool for generating genetically modified animals; however, targeted knock-in (KI) via homologous recombination remains difficult in zygotes. Here we show efficient gene KI in rats by combining CRISPR-Cas with single-stranded oligodeoxynucleotides (ssODNs). First,...

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Bibliographic Details
Published in:Nat Commun
Main Authors: Yoshimi, Kazuto, Kunihiro, Yayoi, Kaneko, Takehito, Nagahora, Hitoshi, Voigt, Birger, Mashimo, Tomoji
Format: Artigo
Language:Inglês
Published: Nature Publishing Group 2016
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4736110/
https://ncbi.nlm.nih.gov/pubmed/26786405
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms10431
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