User-defined single pot mutagenesis using unamplified oligo pools

User-defined mutagenic libraries are fundamental for applied protein engineering workflows. Here we show that unamplified oligo pools can be used to prepare site saturation mutagenesis libraries from plasmid DNA with near-complete coverage of desired mutations and few off-target mutations. We find t...

पूर्ण विवरण

में बचाया:
ग्रंथसूची विवरण
में प्रकाशित:Protein Eng Des Sel
मुख्य लेखकों: Medina-Cucurella, Angélica V, Steiner, Paul J, Faber, Matthew S, Beltrán, Jesús, Borelli, Alexandra N, Kirby, Monica B, Cutler, Sean R, Whitehead, Timothy A
स्वरूप: Artigo
भाषा:Inglês
प्रकाशित: Oxford University Press 2019
विषय:
Short Communication
ऑनलाइन पहुंच:https://ncbi.nlm.nih.gov/pmc/articles/PMC7212264/
https://ncbi.nlm.nih.gov/pubmed/31297523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzz013
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