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A Method for User-defined Mutagenesis by Integrating Oligo Pool Synthesis Technology with Nicking Mutagenesis

Saturation mutagenesis is a fundamental enabling technology for protein engineering and epitope mapping. Nicking mutagenesis (NM) allows the user to rapidly construct libraries of all possible single mutations in a target protein sequence from plasmid DNA in a one-pot procedure. Briefly, one strand...

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Bibliografiske detaljer
Udgivet i:Bio Protoc
Main Authors: Steiner, Paul J., Baumer, Zachary T., Whitehead, Timothy A.
Format: Artigo
Sprog:Inglês
Udgivet: Bio-Protocol 2020
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842585/
https://ncbi.nlm.nih.gov/pubmed/33659364
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3697
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