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PFunkel: Efficient, Expansive, User-Defined Mutagenesis
We introduce PFunkel, a versatile method for extensive, researcher-defined DNA mutagenesis using a ssDNA or dsDNA template. Once the template DNA is prepared, the method can be completed in a single day in a single tube, and requires no intermediate DNA purification or sub-cloning. PFunkel can be us...
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Public Library of Science
2012
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3524131/ https://ncbi.nlm.nih.gov/pubmed/23284860 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0052031 |
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