User-defined single pot mutagenesis using unamplified oligo pools

User-defined mutagenic libraries are fundamental for applied protein engineering workflows. Here we show that unamplified oligo pools can be used to prepare site saturation mutagenesis libraries from plasmid DNA with near-complete coverage of desired mutations and few off-target mutations. We find t...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Protein Eng Des Sel
Prif Awduron: Medina-Cucurella, Angélica V, Steiner, Paul J, Faber, Matthew S, Beltrán, Jesús, Borelli, Alexandra N, Kirby, Monica B, Cutler, Sean R, Whitehead, Timothy A
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2019
Pynciau:
Short Communication
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7212264/
https://ncbi.nlm.nih.gov/pubmed/31297523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzz013
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