User-defined single pot mutagenesis using unamplified oligo pools

User-defined mutagenic libraries are fundamental for applied protein engineering workflows. Here we show that unamplified oligo pools can be used to prepare site saturation mutagenesis libraries from plasmid DNA with near-complete coverage of desired mutations and few off-target mutations. We find t...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:Protein Eng Des Sel
Autori principali: Medina-Cucurella, Angélica V, Steiner, Paul J, Faber, Matthew S, Beltrán, Jesús, Borelli, Alexandra N, Kirby, Monica B, Cutler, Sean R, Whitehead, Timothy A
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2019
Soggetti:
Short Communication
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7212264/
https://ncbi.nlm.nih.gov/pubmed/31297523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzz013
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