User-defined single pot mutagenesis using unamplified oligo pools

User-defined mutagenic libraries are fundamental for applied protein engineering workflows. Here we show that unamplified oligo pools can be used to prepare site saturation mutagenesis libraries from plasmid DNA with near-complete coverage of desired mutations and few off-target mutations. We find t...

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Publicat a:Protein Eng Des Sel
Autors principals: Medina-Cucurella, Angélica V, Steiner, Paul J, Faber, Matthew S, Beltrán, Jesús, Borelli, Alexandra N, Kirby, Monica B, Cutler, Sean R, Whitehead, Timothy A
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2019
Matèries:
Short Communication
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7212264/
https://ncbi.nlm.nih.gov/pubmed/31297523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzz013
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