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Characterizing Protein-Protein Interactions Using Deep Sequencing Coupled to Yeast Surface Display
In this chapter, we discuss a method to determine the affinity and specificity of nearly all single-point mutants for a full-length protein binder. This method combines deep sequencing, comprehensive mutagenesis, yeast surface display, and fluorescence-activated cell sorting. This approach has been...
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| Udgivet i: | Methods Mol Biol |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2018
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6664802/ https://ncbi.nlm.nih.gov/pubmed/29605911 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7759-8_7 |
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