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Pro region engineering of nerve growth factor by deep mutational scanning enables a yeast platform for conformational epitope mapping of anti-NGF monoclonal antibodies

Nerve growth factor (NGF) plays a central role in multiple chronic pain conditions. As such, anti-NGF monoclonal antibodies (mAbs) that function by antagonizing NGF downstream signaling are leading drug candidates for non-opioid pain relief. To evaluate anti-canine NGF (cNGF) mAbs we sought a yeast...

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Publicat a:Biotechnol Bioeng
Autors principals: Medina-Cucurella, Angélica V., Zhu, Yaqi, Bowen, Scott J., Bergeron, Lisa M., Whitehead, Timothy A.
Format: Artigo
Idioma:Inglês
Publicat: 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6664808/
https://ncbi.nlm.nih.gov/pubmed/29663315
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.26706
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