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Identification of protein/target molecule interactions using yeast surface-displayed cDNA libraries
We describe a novel expression cloning method based on screening yeast surface-displayed human cDNA libraries by direct affinity interaction to identify cellular proteins binding to a broad spectrum of target molecules. Being a eukaryote, yeast protein expression pathways are similar to those found...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3229213/ https://ncbi.nlm.nih.gov/pubmed/21365493 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-065-2_14 |
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