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Saturation Mutagenesis Genome Engineering of Infective ΦX174 Bacteriophage via Unamplified Oligo Pools and Golden Gate Assembly
Here we present a novel protocol for the construction of saturation single-site—and massive multisite—mutant libraries of a bacteriophage. We segmented the ΦX174 genome into 14 nontoxic and nonreplicative fragments compatible with Golden Gate assembly. We next used nicking mutagenesis with oligonucl...
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| Publicado no: | ACS Synth Biol |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7055157/ https://ncbi.nlm.nih.gov/pubmed/31825605 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.9b00411 |
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