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Plasmid-based one-pot saturation mutagenesis
Deep mutational scanning is a foundational tool for addressing the functional consequences of large numbers of mutants, but a more efficient and accessible method for construction of user-defined mutagenesis libraries is needed. Here we present nicking mutagenesis, a robust, single-day, one-pot satu...
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| Publicado no: | Nat Methods |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5666567/ https://ncbi.nlm.nih.gov/pubmed/27723752 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4029 |
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