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An automated data-driven pipeline for improving heterologous enzyme expression

Enzymes are the ultimate entities responsible for chemical transformations in natural and engineered biosynthetic pathways. However, many natural enzymes suffer from suboptimal functional expression due to poor intrinsic protein stability. Further, stability enhancing mutations often come at the cos...

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Bibliographic Details
Published in:ACS Synth Biol
Main Authors: Wrenbeck, Emily E., Bedewitz, Matthew A., Klesmith, Justin R., Noshin, Syeda, Barry, Cornelius S., Whitehead, Timothy A.
Format: Artigo
Language:Inglês
Published: 2019
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6855305/
https://ncbi.nlm.nih.gov/pubmed/30721031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.8b00486
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