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An automated data-driven pipeline for improving heterologous enzyme expression

Enzymes are the ultimate entities responsible for chemical transformations in natural and engineered biosynthetic pathways. However, many natural enzymes suffer from suboptimal functional expression due to poor intrinsic protein stability. Further, stability enhancing mutations often come at the cos...

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Detalles Bibliográficos
Publicado en:ACS Synth Biol
Autores principales: Wrenbeck, Emily E., Bedewitz, Matthew A., Klesmith, Justin R., Noshin, Syeda, Barry, Cornelius S., Whitehead, Timothy A.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2019
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6855305/
https://ncbi.nlm.nih.gov/pubmed/30721031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.8b00486
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