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Trade-offs between enzyme fitness and solubility illuminated by deep mutational scanning

Proteins are marginally stable, and an understanding of the sequence determinants for improved protein solubility is highly desired. For enzymes, it is well known that many mutations that increase protein solubility decrease catalytic activity. These competing effects frustrate efforts to design and...

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Publicat a:Proc Natl Acad Sci U S A
Autors principals: Klesmith, Justin R., Bacik, John-Paul, Wrenbeck, Emily E., Michalczyk, Ryszard, Whitehead, Timothy A.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5338495/
https://ncbi.nlm.nih.gov/pubmed/28196882
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1614437114
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