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Trade-offs between enzyme fitness and solubility illuminated by deep mutational scanning

Proteins are marginally stable, and an understanding of the sequence determinants for improved protein solubility is highly desired. For enzymes, it is well known that many mutations that increase protein solubility decrease catalytic activity. These competing effects frustrate efforts to design and...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Klesmith, Justin R., Bacik, John-Paul, Wrenbeck, Emily E., Michalczyk, Ryszard, Whitehead, Timothy A.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5338495/
https://ncbi.nlm.nih.gov/pubmed/28196882
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1614437114
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