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Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded

Our lack of total understanding of the intricacies of how enzymes behave has constrained our ability to robustly engineer substrate specificity. Furthermore, the mechanisms of natural evolution leading to improved or novel substrate specificities are not wholly defined. Here we generate near-compreh...

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Bibliografski detalji
Izdano u:Nat Commun
Glavni autori: Wrenbeck, Emily E., Azouz, Laura R., Whitehead, Timothy A.
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5467163/
https://ncbi.nlm.nih.gov/pubmed/28585537
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms15695
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