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An efficient one-step site-directed and site-saturation mutagenesis protocol
We have developed a new primer design method based on the QuickChange™ site-directed mutagenesis protocol, which significantly improves the PCR amplification efficiency. This design method minimizes primer dimerization and ensures the priority of primer-template annealing over primer self-pairing du...
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| 主要な著者: | , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
2004
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC514394/ https://ncbi.nlm.nih.gov/pubmed/15304544 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh110 |
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