An efficient one-step site-directed and site-saturation mutagenesis protocol
We have developed a new primer design method based on the QuickChange™ site-directed mutagenesis protocol, which significantly improves the PCR amplification efficiency. This design method minimizes primer dimerization and ensures the priority of primer-template annealing over primer self-pairing du...
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| Yayımlandı: | Nucleic Acids Res |
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| Asıl Yazarlar: | , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Oxford University Press
2004
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC514394/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15304544/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh110 |
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