Site-Directed Mutagenesis of Large Plasmids
A protocol combining recombination PCR and long-distance PCR is demonstrated to be highly accurate and rapid for sitedirected mutagenesis of large (>10 kb) plasmids. Application of this protocol to the generation of mutant rabies virus glycoproteins expressed by the baculovirus/insect cell system il...
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| Principais autores: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis Group
1998-12-01
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| coleção: | BioTechniques |
| Acesso em linha: | https://www.future-science.com/doi/10.2144/98256st04 |
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