A simple and economical site-directed mutagenesis method for large plasmids by direct transformation of two overlapping PCR fragments

Despite the development of various methods and commercial kits, site-directed mutagenesis of large plasmids remains a challenge in many laboratories. A site-directed mutagenesis method was developed for large plasmids by directly transforming two overlapping PCR fragments into Escherichia coli. This...

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Detalhes bibliográficos

Principais autores:	Zhibo Yang, Zan Chen, Yueping Zhang
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Taylor & Francis Group 2022-11-01
coleção:	BioTechniques
Assuntos:	Escherichia coli Gibson assembly in vivo assembly in vivo recombination overlapping extension PCR PCR-based site-directed mutagenesis
Acesso em linha:	https://www.future-science.com/doi/10.2144/btn-2022-0085
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