Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.

A mutation of a cloned gene that has been made by introducing a transposon or some other selectable genetic determinant can be crossed into the gene's original replicon by linearizing the cloned DNA and transforming a recB recC sbcB mutant. A number of applications of this method are described with...

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Detalles Bibliográficos

Publicado en:	J Bacteriol
Principais autores:	Winans, S C, Elledge, S J, Krueger, J H, Walker, G C
Formato:	Artigo
Idioma:	Inglês
Publicado:	American Society for Microbiology (ASM) 1985
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215030/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2982787/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.161.3.1219-1221.1985
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