Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.

A mutation of a cloned gene that has been made by introducing a transposon or some other selectable genetic determinant can be crossed into the gene's original replicon by linearizing the cloned DNA and transforming a recB recC sbcB mutant. A number of applications of this method are described with...

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Pubblicato in:	J Bacteriol
Autori principali:	Winans, S C, Elledge, S J, Krueger, J H, Walker, G C
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1985
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215030/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2982787/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.161.3.1219-1221.1985
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