Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.
A mutation of a cloned gene that has been made by introducing a transposon or some other selectable genetic determinant can be crossed into the gene's original replicon by linearizing the cloned DNA and transforming a recB recC sbcB mutant. A number of applications of this method are described with...
שמור ב:
| הוצא לאור ב: | J Bacteriol |
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| Principais autores: | , , , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
American Society for Microbiology (ASM)
1985
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215030/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2982787/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.161.3.1219-1221.1985 |
| תגים: |
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