An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases

Proteins fused to the elastin-like polypeptide (ELP) tag can be selectively separated from crude cell extract without chromatography. To avoid the interference of the ELP tag on properties of the target protein, it is necessary to remove the ELP tag from target protein by protease digestion. Therefo...

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Publicado no:Anal Biochem
Main Authors: Lan, Dongming, Huang, Guangrui, Shao, Hongwei, Zhang, Lichun, Ma, Lixin, Chen, Shangwu, Xu, Anlong
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier Inc. 2011
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7094418/
https://ncbi.nlm.nih.gov/pubmed/21600185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.04.034
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