Purification of Escherichia coli RNA polymerase using a self-cleaving elastin-like polypeptide tag

A self-cleaving elastin-like polypeptide (ELP) tag was used to purify the multisubunit Escherichia coli RNA polymerase (RNAP) via a simple, nonchromatographic method. To accomplish this, the RNAP α subunit was tagged with a self-cleaving ELP-intein tag and coexpressed with the β, β′, and ω subunits....

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Main Authors: Fong, Baley A, Gillies, Alison R, Ghazi, Iraj, LeRoy, Gary, Lee, Kathleen C, Westblade, Lars F, Wood, David W
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2010
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2895248/
https://ncbi.nlm.nih.gov/pubmed/20512976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.403
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