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Purification of Escherichia coli RNA polymerase using a self-cleaving elastin-like polypeptide tag
A self-cleaving elastin-like polypeptide (ELP) tag was used to purify the multisubunit Escherichia coli RNA polymerase (RNAP) via a simple, nonchromatographic method. To accomplish this, the RNAP α subunit was tagged with a self-cleaving ELP-intein tag and coexpressed with the β, β′, and ω subunits....
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Wiley Subscription Services, Inc., A Wiley Company
2010
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2895248/ https://ncbi.nlm.nih.gov/pubmed/20512976 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.403 |
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