Improved Non-Chromatographic Purification of a Recombinant Protein by Cationic Elastin-like Polypeptides

This paper reports an improvement in the purification of thioredoxin (Trx) expressed from E. coli by inverse transition cycling (ITC) using cationic elastin-like polypeptides (ELPs). Two ELP libraries having 2 and 5 % lysine residues and molecular weights ranging from 4 to 61.1 kDa showed greater sa...

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Bibliografiske detaljer
Main Authors: Lim, Dong Woo, Trabbic-Carlson, Kimberly, MacKay, J. Andrew, Chilkoti, Ashutosh
Format: Artigo
Sprog:Inglês
Udgivet: 2007
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2562536/
https://ncbi.nlm.nih.gov/pubmed/17407348
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bm060849t
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