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Improved Non-Chromatographic Purification of a Recombinant Protein by Cationic Elastin-like Polypeptides
This paper reports an improvement in the purification of thioredoxin (Trx) expressed from E. coli by inverse transition cycling (ITC) using cationic elastin-like polypeptides (ELPs). Two ELP libraries having 2 and 5 % lysine residues and molecular weights ranging from 4 to 61.1 kDa showed greater sa...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2007
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2562536/ https://ncbi.nlm.nih.gov/pubmed/17407348 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bm060849t |
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