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Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

Elastin-like polypeptides are repetitive biopolymers that exhibit a lower critical solution temperature phase transition behavior, existing as soluble unimers below a characteristic transition temperature and aggregating into micron-scale coacervates above their transition temperature. The design of...

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Bibliografiske detaljer
Main Authors: MacEwan, Sarah R., Hassouneh, Wafa, Chilkoti, Ashutosh
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4188175/
https://ncbi.nlm.nih.gov/pubmed/24961229
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51583
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