An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases

Proteins fused to the elastin-like polypeptide (ELP) tag can be selectively separated from crude cell extract without chromatography. To avoid the interference of the ELP tag on properties of the target protein, it is necessary to remove the ELP tag from target protein by protease digestion. Therefo...

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Pubblicato in:Anal Biochem
Autori principali: Lan, Dongming, Huang, Guangrui, Shao, Hongwei, Zhang, Lichun, Ma, Lixin, Chen, Shangwu, Xu, Anlong
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier Inc. 2011
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7094418/
https://ncbi.nlm.nih.gov/pubmed/21600185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.04.034
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