An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases

Proteins fused to the elastin-like polypeptide (ELP) tag can be selectively separated from crude cell extract without chromatography. To avoid the interference of the ELP tag on properties of the target protein, it is necessary to remove the ELP tag from target protein by protease digestion. Therefo...

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Argitaratua izan da:Anal Biochem
Egile Nagusiak: Lan, Dongming, Huang, Guangrui, Shao, Hongwei, Zhang, Lichun, Ma, Lixin, Chen, Shangwu, Xu, Anlong
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Elsevier Inc. 2011
Gaiak:
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Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC7094418/
https://ncbi.nlm.nih.gov/pubmed/21600185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.04.034
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