Nalaganje...
“Three-in-one” chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using Sortase A and elastin-like polypeptides
Applied in tandem, elastin-like polypeptides (ELPs) and the sortase A (SrtA) transpeptidase from Staphylococcus aureus provide a general method for chromatography-free purification of tag-free recombinant proteins and optional, site-specific and homogeneous conjugation of the protein to a small mole...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2013
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3723126/ https://ncbi.nlm.nih.gov/pubmed/23424160 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201208292 |
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