Wird geladen...
“Three-in-one” chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using Sortase A and elastin-like polypeptides
Applied in tandem, elastin-like polypeptides (ELPs) and the sortase A (SrtA) transpeptidase from Staphylococcus aureus provide a general method for chromatography-free purification of tag-free recombinant proteins and optional, site-specific and homogeneous conjugation of the protein to a small mole...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2013
|
| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3723126/ https://ncbi.nlm.nih.gov/pubmed/23424160 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201208292 |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|