Expression, Purification, and Functional Reconstitution of (19)F-labeled Cytochrome b5 in Peptide Nanodiscs for NMR Studies

Microsomal cytochrome b5 (cytb5) is a membrane-bound protein capable of donating the second electron to cytochrome P450s (cytP450s) in the cytP450s monooxygenase reactions. Recent studies have demonstrated the importance of the transmembrane domain of cytb5 in the interaction with cytP450 by stabili...

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Udgivet i:Biochim Biophys Acta Biomembr
Main Authors: Bai, Jia, Wang, Jian, Ravula, Thirupathi, Im, Sang-Choul, Anantharamaiah, G. M., Waskell, Lucy, Ramamoorthy, Ayyalusamy
Format: Artigo
Sprog:Inglês
Udgivet: 2020
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7050362/
https://ncbi.nlm.nih.gov/pubmed/31953231
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbamem.2020.183194
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