CRISPR-Cas12a Possesses Unconventional DNase Activity that Can Be Inactivated by Synthetic Oligonucleotides

CRISPR-Cas12a (CRISPR-Cpf1) was reported to have multiple types of cleavage activities. Without the assistance of CRISPR RNA (crRNA), we investigated DNase activity and substrate specificity of Cas12a orthologs in the presence of diverse divalent metal ions. Cas12a from different species are capable...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Mol Ther Nucleic Acids
Prif Awduron: Li, Bin, Yan, Jingyue, Zhang, Youxi, Li, Wenqing, Zeng, Chunxi, Zhao, Weiyu, Hou, Xucheng, Zhang, Chengxiang, Dong, Yizhou
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society of Gene & Cell Therapy 2020
Pynciau:
Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7015830/
https://ncbi.nlm.nih.gov/pubmed/32045875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtn.2019.12.038
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