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CRISPR-Cas12a Possesses Unconventional DNase Activity that Can Be Inactivated by Synthetic Oligonucleotides

CRISPR-Cas12a (CRISPR-Cpf1) was reported to have multiple types of cleavage activities. Without the assistance of CRISPR RNA (crRNA), we investigated DNase activity and substrate specificity of Cas12a orthologs in the presence of diverse divalent metal ions. Cas12a from different species are capable...

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Detalhes bibliográficos
Publicado no:Mol Ther Nucleic Acids
Main Authors: Li, Bin, Yan, Jingyue, Zhang, Youxi, Li, Wenqing, Zeng, Chunxi, Zhao, Weiyu, Hou, Xucheng, Zhang, Chengxiang, Dong, Yizhou
Formato: Artigo
Idioma:Inglês
Publicado em: American Society of Gene & Cell Therapy 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7015830/
https://ncbi.nlm.nih.gov/pubmed/32045875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtn.2019.12.038
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