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A Liquid Chromatography/Mass Spectrometry Method for Screening Disulfide Tethering Fragments

We report the refinement of a high-throughput, liquid chromatography/mass spectrometry (LC/MS)–based screening method for the identification of covalent small-molecule binders to proteins. Using a custom library of 1600 disulfide-capped fragments targeting surface cysteine residues, we optimize samp...

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Publicat a:SLAS Discov
Autors principals: Hallenbeck, Kenneth K., Davies, Julia L., Merron, Connie, Ogden, Pierce, Sijbesma, Eline, Ottmann, Christian, Renslo, Adam R., Wilson, Christopher, Arkin, Michelle R.
Format: Artigo
Idioma:Inglês
Publicat: 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6609441/
https://ncbi.nlm.nih.gov/pubmed/28945980
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/2472555217732072
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