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Accurate Sequence Analysis of a Monoclonal Antibody by Top-Down and Middle-Down Orbitrap Mass Spectrometry Applying Multiple Ion Activation Techniques

Targeted top-down (TD) and middle-down (MD) mass spectrometry (MS) offer reduced sample manipulation during protein analysis, limiting the risk of introducing artifactual modifications to better capture sequence information on the proteoforms present. This provides some advantages when characterizin...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Anal Chem
Hauptverfasser: Fornelli, Luca, Srzentić, Kristina, Huguet, Romain, Mullen, Christopher, Sharma, Seema, Zabrouskov, Vlad, Fellers, Ryan T., Durbin, Kenneth R., Compton, Philip D., Kelleher, Neil L.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6071867/
https://ncbi.nlm.nih.gov/pubmed/29894161
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.8b00984
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