Easy quantification of template-directed CRISPR/Cas9 editing

Template-directed CRISPR/Cas9 editing is a powerful tool for introducing subtle mutations in genomes. However, the success rate of incorporation of the desired mutations at the target site is difficult to predict and therefore must be empirically determined. Here, we adapted the widely used TIDE met...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Brinkman, Eva K, Kousholt, Arne N, Harmsen, Tim, Leemans, Christ, Chen, Tao, Jonkers, Jos, van Steensel, Bas
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2018
Fag:
Methods Online
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6007333/
https://ncbi.nlm.nih.gov/pubmed/29538768
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky164
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