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Easy quantification of template-directed CRISPR/Cas9 editing

Template-directed CRISPR/Cas9 editing is a powerful tool for introducing subtle mutations in genomes. However, the success rate of incorporation of the desired mutations at the target site is difficult to predict and therefore must be empirically determined. Here, we adapted the widely used TIDE met...

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Bibliografiska uppgifter
I publikationen:Nucleic Acids Res
Huvudupphovsmän: Brinkman, Eva K, Kousholt, Arne N, Harmsen, Tim, Leemans, Christ, Chen, Tao, Jonkers, Jos, van Steensel, Bas
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2018
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC6007333/
https://ncbi.nlm.nih.gov/pubmed/29538768
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky164
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