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Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9

BACKGROUND: Microbial genome editing is a powerful tool to modify chromosome in way of deletion, insertion or replacement, which is one of the most important techniques in metabolic engineering research. The emergence of CRISPR/Cas9 technique inspires various genomic editing methods. RESULTS: In thi...

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Detalhes bibliográficos
Publicado no:Microb Cell Fact
Main Authors: Zhao, Dongdong, Yuan, Shenli, Xiong, Bin, Sun, Hongnian, Ye, Lijun, Li, Jing, Zhang, Xueli, Bi, Changhao
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5134288/
https://ncbi.nlm.nih.gov/pubmed/27908280
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0605-5
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