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A programmable CRISPR/Cas9-based phage defense system for Escherichia coli BL21(DE3)

Escherichia coli BL21 is arguably the most popular host for industrial production of proteins, and industrial fermentations are often plagued by phage infections. The CRISPR/Cas system is guided by a gRNA to cleave a specific DNA cassette, which can be developed into a highly efficient programable p...

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Dettagli Bibliografici
Pubblicato in:Microb Cell Fact
Autori principali: Liu, Li, Zhao, Dongdong, Ye, Lijun, Zhan, Tao, Xiong, Bin, Hu, Muzi, Bi, Changhao, Zhang, Xueli
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7332536/
https://ncbi.nlm.nih.gov/pubmed/32620105
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-020-01393-2
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