Easy quantification of template-directed CRISPR/Cas9 editing

Template-directed CRISPR/Cas9 editing is a powerful tool for introducing subtle mutations in genomes. However, the success rate of incorporation of the desired mutations at the target site is difficult to predict and therefore must be empirically determined. Here, we adapted the widely used TIDE met...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Brinkman, Eva K, Kousholt, Arne N, Harmsen, Tim, Leemans, Christ, Chen, Tao, Jonkers, Jos, van Steensel, Bas
Format: Artigo
Language:Inglês
Published: Oxford University Press 2018
Subjects:
Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6007333/
https://ncbi.nlm.nih.gov/pubmed/29538768
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky164
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