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Easy quantification of template-directed CRISPR/Cas9 editing

Template-directed CRISPR/Cas9 editing is a powerful tool for introducing subtle mutations in genomes. However, the success rate of incorporation of the desired mutations at the target site is difficult to predict and therefore must be empirically determined. Here, we adapted the widely used TIDE met...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Brinkman, Eva K, Kousholt, Arne N, Harmsen, Tim, Leemans, Christ, Chen, Tao, Jonkers, Jos, van Steensel, Bas
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6007333/
https://ncbi.nlm.nih.gov/pubmed/29538768
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky164
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