Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

An efficient genome-scale editing tool is required for construction of industrially useful microbes. We describe a targeted, continual multigene editing strategy that was applied to the Escherichia coli genome by using the Streptococcus pyogenes type II CRISPR-Cas9 system to realize a variety of pre...

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Bibliographic Details
Published in:Appl Environ Microbiol
Main Authors: Jiang, Yu, Chen, Biao, Duan, Chunlan, Sun, Bingbing, Yang, Junjie, Yang, Sheng
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2015
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4357945/
https://ncbi.nlm.nih.gov/pubmed/25636838
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.04023-14
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